Pure

The 3M Molecular Detection System uses multiple, specific primers targeting distinct regions of the genome, combined with real-time detection of the amplification, to deliver sensitive and specific results.

Continuous amplification by a unique, high-fidelity DNA polymerase makes our system less prone to matrix interference. This was designed to help you perform fewer repeat tests and make critical decisions faster.

Simple

Our system makes testing easy, increasing technician productivity and efficiency. All you need is an enriched sample, a laptop and the 3M Molecular Detection System. Using a DNA polymerase that is tolerant to low-quality DNA samples allows for a simple assay set-up with fewer steps.

Your lab can even test and display results for multiple organisms in a single run. The simplicity and robustness of our technology combine to deliver a low-cost instrument with minimal maintenance requirements and less downtime for your lab.

 

 

 

 

 

How does it work?

The 3M Molecular Detection System uses multiple primers to recognize distinct regions of the genome and Bst DNA polymerase to provide continuous and rapid amplification of genetic material.

Pyrophosphate ions (PPi), a by-product of the targeted DNA amplification reaction, and APS, are enzymatically converted into ATP by ATP-Sulfurylase. ATP reacts with luciferase to produce light which is detected indicating the presence of target organism DNA.

Both amplification and detection occur simultaneously and continuously during the exponential phase providing real time results and a short run time.

Accurate

Multiple, specific primers target distinct regions of the genome resulting in more efficient amplification of target DNA and accurate results

Best DNA polymerase is less susceptible to inhibiting substances found in food samples than other polymerases

DNA is amplified continuously under isothermal conditions limiting the possible interference of the template or DNA polymerase by inhibitors found in food samples

Detection occurs during the exponential phase of amplification enhancing specificity

Bioluminescence detection has a better signal-to-noise ratio than fluorescence and is not affected by interference from fluorophores that can be present in some foods (ex. food dyes, acriflavin in enrichment media, etc.)

Fast and Simple

The continuous DNA strand displacement process enables efficient and rapid amplification of DNA

Bioluminescence provides real-time detection of the DNA amplification AND simultaneous amplification and detection allows for detection of positive results before the end of the run

The DNA polymerase is robust and tolerant to low-quality DNA allowing a simple sample preparation and an assay set up with fewer steps

Bioluminescence has a better signal-to-noise ratio than fluorescence and is not affected by interference from other fluorophores making the process more robust and allowing the same procedure to be followed for all assays

Because the DNA amplification is detected via bioluminescence, the 3M Molecular Detection System offers the unique use of color-coded assay tubes to differentiate pathogen assays

Cost Effective

Isothermal DNA amplification proceeds at a constant temperature, removing the need for complicated instrumentation

Bioluminescence detection eliminates the need for high-cost excitation sources, fluorophores, fluorescent filters and detectors resulting in a robust instrument with minimal maintenance requirements

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